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Risperidone Toxicity on Human Blood Lymphocytes in Nano molar Concentrations
Bahareh Sadat Yousefsani1,2, Ahmad Salimi3, Farnaz Imani4
1Research Institute for Islamic and Complementary Medicine, Iran University of Medical Sciences, Tehran, Iran.
Drug Research
|May 23, 2022
Summary
Risperidone, an antipsychotic, causes toxicity in human lymphocytes by increasing oxidative stress. This involves elevated reactive oxygen species (ROS) and impaired cellular functions.
Area of Science:
- Immunology
- Pharmacology
- Toxicology
Background:
- Risperidone is an atypical antipsychotic used for psychiatric disorders.
- Emerging evidence suggests risperidone may exhibit toxicity to the immune system.
Purpose of the Study:
- To evaluate the toxic effects of risperidone on human blood lymphocytes.
- To investigate the underlying mechanisms of risperidone-induced lymphocyte toxicity.
Main Methods:
- Human blood lymphocytes were isolated using Ficoll Paque Plus.
- Lymphocytes were exposed to varying concentrations of risperidone (12, 24, 48 nM).
- Key indicators of cellular toxicity, including reactive oxygen species (ROS), lysosomal membrane integrity, mitochondrial membrane potential (MMP), and glutathione (GSH/GSSG) levels, were assessed.
Main Results:
- Risperidone exposure significantly increased intracellular reactive oxygen species (ROS) levels.
- Damage to the lysosomal membrane and collapse of mitochondrial membrane potential (MMP) were observed.
- Extracellular oxidized glutathione (GSSG) increased, while intracellular glutathione (GSH) decreased, indicating oxidative stress.
Conclusions:
- Oxidative stress is a key mechanism contributing to risperidone's toxicity in human blood lymphocytes.
- Risperidone disrupts critical cellular functions within lymphocytes, potentially impacting immune response.
- These findings highlight the need for careful monitoring of immune system effects during risperidone pharmacotherapy.
