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Microalgae Cultivation and Biomass Quantification in a Bench-Scale Photobioreactor with Corrosive Flue Gases

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An open-source, automated, gas sampling peripheral for laboratory incubation experiments using cavity ring-down

Timothy D Berry¹, Chance Creelman², Nick Nickerson²

¹Department of Soil Science, University of Wisconsin-Madison, United States.

|May 24, 2022

Summary

Researchers developed a scalable gas sampling multiplexer for autonomous analysis of up to 56 samples. This automated system enhances isotopic composition and gas flux measurements in various spectroscopic instruments.

Keywords:

Autosampler Cavity ring-down spectroscopy Gas sampling Respiration study Soil gas fluxes Trace gas analysis

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Area of Science:

Environmental Science
Analytical Chemistry
Instrumentation

Background:

Spectroscopic instruments are crucial for analyzing gas isotopic composition and fluxes.
Existing automated gas sampling peripherals (multiplexers) are limited to a small number of samples (≤16).
This limitation hinders studies requiring high-throughput or large-scale sample analysis.

Purpose of the Study:

To design and present a scalable, automated gas sampling peripheral (multiplexer).
To enable autonomous sampling of up to 56 vessels for spectroscopic analysis.
To provide a flexible platform adaptable to various gas analysis instruments.

Main Methods:

Development of a custom-designed, programmable gas sampling multiplexer.
Integration with a Picarro cavity ring-down spectroscopy (CRDS) system for demonstration.
Autonomous headspace CO2 sampling from 14 laboratory-incubated soils amended with 13C-enriched pyrogenic organic matter.

Main Results:

Successful demonstration of autonomous gas sampling capabilities for up to 56 vessels.
The multiplexer effectively sampled CO2 from soil incubation experiments for isotopic analysis.
The design and data processing approach are adaptable to diverse spectroscopic and gas analysis platforms.

Conclusions:

The presented scalable multiplexer overcomes limitations of commercial systems, supporting larger sample throughput.
The system is versatile and can be adapted for various gas sampling and delivery applications.
This automated solution facilitates advanced isotopic analysis in environmental and laboratory studies.