Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Genomic Imprinting and Inheritance02:30

Genomic Imprinting and Inheritance

35.3K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
35.3K
General Transcription Factors01:30

General Transcription Factors

5.7K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.7K
Transcription Factors02:16

Transcription Factors

78.0K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
78.0K
Cellular Differentiation00:57

Cellular Differentiation

3.8K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
3.8K
Epigenetic Regulation01:46

Epigenetic Regulation

31.4K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
31.4K
Master Transcription Regulators02:23

Master Transcription Regulators

7.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of agricultural pesticides on survival, behavior, and reproductive capacity in Drosophila melanogaster at real-world exposure levels.

Journal of environmental management·2025
Same author

Harnessing spatial transcriptomics to understand host-parasite interactions in plants and animals.

Biotechnology advances·2025
Same author

RNA tomography reveals spatial gene expression maps of Arabidopsis thaliana roots infected with Heterodera schachtii.

The New phytologist·2025
Same author

Lamin variants cause cardiac arrhythmogenicity in Drosophila.

Disease models & mechanisms·2025
Same author

Geranylgeranylacetone as Prevention for Postoperative Atrial Fibrillation (GENIALITY).

Cardiovascular drugs and therapy·2025
Same author

Systemic coordination of whole-body tissue remodeling during local regeneration in sea anemones.

Developmental cell·2024

Related Experiment Video

Updated: Sep 22, 2025

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

18.2K

Endomitosis controls tissue-specific gene expression during development.

Lotte M van Rijnberk1, Ramon Barrull-Mascaró1, Reinier L van der Palen1

  • 1Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences and University Medical Center Utrecht, Utrecht, the Netherlands.

Plos Biology
|May 24, 2022
PubMed
Summary

Multinucleation in C. elegans intestinal cells drives rapid gene expression during development. This process is crucial for nutrient transport, with disruptions impacting progeny development and fitness.

More Related Videos

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
08:43

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Published on: June 6, 2016

9.0K
Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
14:08

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Published on: January 26, 2013

15.4K

Related Experiment Videos

Last Updated: Sep 22, 2025

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

18.2K
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
08:43

Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos

Published on: June 6, 2016

9.0K
Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
14:08

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Published on: January 26, 2013

15.4K

Area of Science:

  • Cell Biology
  • Developmental Biology
  • Genomics

Background:

  • Polyploid cells, with over 2 genome copies, are common in multicellular organisms.
  • Polyploidy can be mononucleated (one nucleus) or multinucleated (multiple nuclei).
  • The functional roles of different polyploidy types remain largely uncharacterized.

Purpose of the Study:

  • To investigate the functional significance of multinucleation in Caenorhabditis elegans intestinal cells.
  • To determine if multinucleation impacts tissue-specific gene expression independently of genome ploidy.

Main Methods:

  • Specific inhibition of binucleation in C. elegans intestinal cells, maintaining ploidy.
  • Single-worm RNA sequencing to analyze gene expression changes.
  • Assessment of progeny development and fitness following altered gene expression.

Main Results:

  • Binucleation is essential for rapid up-regulation of intestine-specific genes during the transition from larval development to adulthood.
  • Key regulated genes include vitellogenins, critical for nutrient transport to the germline.
  • Reduced vitellogenin expression in mononucleated cells resulted in progeny with developmental delays and reduced fitness.

Conclusions:

  • Binucleation facilitates rapid, ploidy-independent gene expression crucial for development.
  • Spatial genome organization within polyploid cells is vital for proper cellular function.
  • Multinucleation plays a significant role in nutrient provisioning for offspring fitness.