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Related Concept Videos

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Related Experiment Video

Updated: Sep 22, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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New adventures in spatial transcriptomics.

Maayan Pour1, Itai Yanai1

  • 1Institute for Computational Medicine, NYU School of Medicine, New York, NY 10016, USA.

Developmental Cell
|May 24, 2022
PubMed
Summary

Spatial transcriptomics, like Stereo-seq, helps untangle complex gene regulatory pathways driving cellular development. This technology reveals how genetic programs transform spatial cell arrangements in distinct biological contexts.

Area of Science:

  • Developmental Biology
  • Genomics
  • Spatial Transcriptomics

Background:

  • Complex biological processes, such as embryonic development, rely on intricate gene regulatory networks.
  • Understanding how these genetic programs orchestrate changes in cellular spatial organization is a fundamental challenge in biology.

Purpose of the Study:

  • To highlight the utility of Stereo-seq, a novel spatial transcriptomics technology.
  • To showcase Stereo-seq's application across diverse biological systems.

Main Methods:

  • Stereo-seq enables high-resolution whole-transcriptome analysis within intact biological tissues.
  • This method captures gene expression patterns with precise spatial localization.

Main Results:

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  • Three independent studies in this issue demonstrate Stereo-seq's effectiveness in distinct biological contexts.
  • The results showcase the method's power to reveal spatial gene expression dynamics.

    • Conclusions:

    • Stereo-seq is a powerful and versatile tool for dissecting gene regulatory mechanisms in development.
    • This technology significantly advances our ability to study spatial genomics and cellular organization.