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Related Experiment Video

Updated: Sep 22, 2025

Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials
08:53

Quantifying Three-Dimensional Cell Migration Within and Into Granular Hydrogel Biomaterials

Published on: March 7, 2025

765

Granular Matrigel: restructuring a trusted extracellular matrix material for improved permeability.

Zahra Mahdieh1,2, Michelle D Cherne3, Jacob P Fredrikson1,2

  • 1Department of Chemical and Biological Engineering, Montana State University, Bozeman, MT, United States of America.

Biomedical Materials (Bristol, England)
|May 24, 2022
PubMed
Summary

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Impact of Sinus Surgery on Bacteriome Composition in Patients With Chronic Rhinosinusitis With Nasal Polyps.

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Physical, chemical, and structural properties of human gastric organoid-derived mucus.

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Correction: Jacobson et al. Innovative Methodology for Antimicrobial Susceptibility Determination in <i>Mycoplasma</i> Biofilms. <i>Microorganisms</i> 2024, <i>12</i>, 2650.

Microorganisms·2025

Researchers created granular Matrigel, a porous material from microgels, to improve transport for cell culture. This new Matrigel structure enhances nutrient and cell movement, supporting organoid growth and immune cell interactions.

Area of Science:

  • Biomaterials Engineering
  • Tissue Engineering
  • Cell Biology

Background:

  • Matrigel, derived from mouse cancer cells, is a widely used extracellular matrix for 2D and 3D cell cultures, including organoids.
  • Limited transport of molecules and cells within Matrigel restricts its applications and negatively impacts cell growth and viability.
  • Restructuring hydrogels into granular forms can enhance porosity and transport without altering chemical composition.

Purpose of the Study:

  • To develop a novel method for creating granular Matrigel using microfluidics.
  • To evaluate the impact of granular Matrigel structure on transport properties.
  • To assess the suitability of granular Matrigel for supporting organoid culture and co-culture applications.

Main Methods:

  • A drop-based microfluidics technique was employed to structure Matrigel into microscopic microgels.
Keywords:
3D cell cultureMatrigelcell migrationgranular microgelmicrofluidicsorganoids

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  • These microgels were packed to form a three-dimensional, mesoporous granular Matrigel material.
  • Transport of colloidal particles and human dendritic cells (DCs) was measured through the granular Matrigel.

    • Main Results:

    • The microfluidics approach successfully produced granular Matrigel, a mesoporous material composed of packed Matrigel microgels.
    • Granular Matrigel demonstrated enhanced transport of colloidal particles and human dendritic cells compared to conventional Matrigel.
    • The granular Matrigel provided adequate mechanical support for culturing human gastric organoids (HGOs) and co-culturing HGOs with DCs.

    Conclusions:

    • Granular Matrigel, fabricated via microfluidics, offers improved transport properties for biomolecules and cells.
    • This restructured Matrigel supports the culture of complex 3D models like human gastric organoids and facilitates immune cell interactions.
    • Granular Matrigel presents a promising advancement for tissue engineering and regenerative medicine applications.