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Related Experiment Video

Updated: Sep 22, 2025

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
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Coordinated cortical ER remodeling facilitates actomyosin ring assembly.

Dan Zhang1, Tingyi See2

  • 1Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, 117604 Singapore, Singapore; Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, 117543 Singapore, Singapore.

Current Biology : CB
|May 24, 2022
PubMed
Summary

Actomyosin compaction and exocytosis coordinate cortical endoplasmic reticulum remodeling for contractile ring assembly in fission yeast. This process ensures proper cell division by dynamically reshaping ER-PM contacts.

Keywords:
ER-PM contact remodelingER-PM contactsER-eisosome contactsVAPactomyosin forcesactomyosin ring assemblycER remodelingeisosomeexocytosisfission yeast

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The Mechanics of Poro-Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton
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The Mechanics of Poro-Elastic Contractile Actomyosin Networks As a Model System of the Cell Cytoskeleton

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Area of Science:

  • Cell Biology
  • Cytoskeleton Dynamics
  • Membrane Remodeling

Background:

  • Cortical endoplasmic reticulum (cER) forms contacts with the plasma membrane (PM).
  • ER-PM contacts influence actomyosin assembly in fission yeast (Schizosaccharomyces pombe).
  • The mechanism linking ER-PM contact remodeling to actomyosin ring formation is not fully understood.

Purpose of the Study:

  • To elucidate how cells remodel ER-PM contacts during actomyosin ring assembly.
  • To investigate the roles of actomyosin compaction and exocytosis in cER reorganization.
  • To understand the contribution of cER-eisosome contacts to ER shaping.

Main Methods:

  • Live-cell imaging in Schizosaccharomyces pombe.
  • Genetic manipulation of actomyosin components (e.g., myosin II).
  • Perturbation of exocytosis pathways.
  • Analysis of cER morphology and ER-PM contact dynamics.

Main Results:

  • Actomyosin compaction drives remodeling of free tubular cER edges.
  • Exocytosis reorganizes eisosome-bound cER rims by altering cER-PM associations.
  • cER-eisosome contacts reserve tubular cER edges at the lateral cortex.
  • Exocytosis facilitates ring formation by enabling robust cER remodeling, especially in mutants with impaired actomyosin compaction.

Conclusions:

  • Coordinated remodeling of cER by actomyosin forces and exocytosis is essential for proper contractile ring assembly.
  • This study provides mechanistic insights into how cER influences actomyosin ring formation.
  • The findings highlight a dynamic interplay between the ER, PM, and actomyosin cytoskeleton during cell division.