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Related Experiment Video

Updated: Sep 22, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

TP53-inducible putative long noncoding RNAs encode functional polypeptides that suppress cell proliferation.

Wenli Xu^1,2, Chang Liu¹, Bing Deng¹

¹MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.

Genome Research

|May 24, 2022

Summary

This summary is machine-generated.

TP53-regulated long noncoding RNAs (lncRNAs) can encode functional peptides that suppress cell proliferation. This discovery expands the TP53 tumor-suppressor network and offers new cancer therapy targets.

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Area of Science:

Molecular Biology
Genomics
Cancer Research

Background:

Long noncoding RNAs (lncRNAs) can encode polypeptides, a novel class of functional molecules.
The role of these lncRNA-encoded peptides in the TP53 pathway remains largely unknown.

Purpose of the Study:

To investigate if TP53-regulated lncRNAs encode functional peptides.
To explore the biological significance of these peptides in human cell lines and their potential role in cancer.

Main Methods:

Ribosome profiling and RNA-sequencing (RNA-seq) in HepG2 cells.
Mass spectrometry for peptide validation.
Functional assays to assess cell proliferation and cell cycle regulation.

Main Results:

Identified over 300 novel TP53-regulated lncRNAs, with 15 confirmed to encode peptides.
Validated several peptides using mass spectrometry.
Demonstrated that TP53-inducible peptides TP53LC02 and TP53LC04 suppress cell proliferation and TP53LC04 regulates the cell cycle in response to DNA damage.

Conclusions:

TP53-regulated lncRNAs can encode novel functional peptides.
These peptides expand the TP53 tumor-suppressor network.
Identified peptides represent potential novel targets for cancer therapy.