Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coordinated stimulation of axon regenerative and neurodegenerative transcriptional programs by ATF4 following optic nerve injury.

eLife·2026
Same author

Noncanonical Taste Transduction of Sugars Elicits Responses in a Dedicated Subset of Gustatory Afferent Neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2025
Same author

Single-Nuclei Sequencing Reveals a Robust Corticospinal Response to Nearby Axotomy But Overall Insensitivity to Spinal Injury.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2025
Same author

Transcobalamin receptor antibodies in autoimmune vitamin B12 central deficiency.

Science translational medicine·2024
Same author

The Effect of the Coronavirus Disease 2019 Pandemic on Pituitary Surgery.

World neurosurgery·2024
Same author

Widening the Operative Corridor-Evaluating the Transcortical Approach to Giant Falcine Meningiomas.

World neurosurgery·2024

Related Experiment Video

Updated: Sep 22, 2025

Widespread Transduction of Mouse Neocortical Neurons by Subarachnoid Injection of AAV2
07:40

Widespread Transduction of Mouse Neocortical Neurons by Subarachnoid Injection of AAV2

Published on: May 23, 2025

337

Selectively Imaging Cranial Sensory Ganglion Neurons Using AAV-PHP.S.

Andoni I Asencor1, Gennady Dvoryanchikov1, Vivien Makhoul1

  • 1Department of Physiology and Biophysics, University of Miami Miller School of Medicine, Miami, FL 33136.

Eneuro
|May 24, 2022
PubMed
Summary
This summary is machine-generated.

Adeno-associated virus PHP.S effectively targets peripheral sensory neurons in mice, enabling detailed mapping of their connections and functions. This advancement aids in understanding sensory neuron subtypes and their circuits.

Keywords:
AAV-PHP.Sauricular neuronscalcium imaginglabeling afferent fiberspseudounipolar sensory neuronssomatosensory

More Related Videos

Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
09:36

Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes

Published on: March 23, 2011

13.2K
Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
10:09

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Published on: September 16, 2022

2.8K

Related Experiment Videos

Last Updated: Sep 22, 2025

Widespread Transduction of Mouse Neocortical Neurons by Subarachnoid Injection of AAV2
07:40

Widespread Transduction of Mouse Neocortical Neurons by Subarachnoid Injection of AAV2

Published on: May 23, 2025

337
Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes
09:36

Optical Imaging of Neurons in the Crab Stomatogastric Ganglion with Voltage-sensitive Dyes

Published on: March 23, 2011

13.2K
Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy
10:09

Evaluation and Manipulation of Neural Activity Using Two-Photon Holographic Microscopy

Published on: September 16, 2022

2.8K

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Adeno-associated viruses (AAVs) are crucial neuroscience tools but have limited use for peripheral sensory neurons due to targeting challenges.
  • The AAV-PHP.S capsid shows enhanced tropism for peripheral neurons, offering a potential solution.

Purpose of the Study:

  • To elaborate on the utility of AAV-PHP.S for targeting peripheral sensory neurons.
  • To demonstrate the effectiveness of AAV-PHP.S in mapping sensory neuron identity and connectivity.

Main Methods:

  • Utilized AAV-PHP.S with GFP or mScarlet fluorescent proteins for viral tracing.
  • Injected virus into mouse sensory ganglia (cranial nerves V, VII, IX, X).
  • Employed light sheet microscopy and in vivo Ca2+ imaging for high-resolution visualization and functional analysis.

Main Results:

  • AAV-PHP.S successfully targeted pseudounipolar neurons in mouse cranial nerve ganglia (V, VII, IX, X).
  • High transduction rates (≈66% in geniculate ganglion) were observed, with reporter transport along central and peripheral axons.
  • Demonstrated cell type-specific expression and characterized previously undocumented sensory functions of geniculate ganglion auricular neurons.

Conclusions:

  • AAV-PHP.S is a powerful tool for anatomical and functional mapping of peripheral sensory neurons.
  • Facilitates the study of diverse somatosensory and viscerosensory neuron subtypes defined by single-cell RNA sequencing.
  • Enables high-resolution visualization of neural circuits and receptive fields.