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Updated: Sep 22, 2025

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
Published on: September 27, 2012
Cell-Based Phenotypic Screens to Discover Circadian Clock-Modulating Compounds
Megumi Hatori1, Tsuyoshi Hirota2
1Institute of Transformative Bio-Molecules, Nagoya University, Nagoya, Japan.
Abstract:
There is increasing demand to control circadian clock functions in a conditional manner for deeper understanding of the circadian system as well as for potential treatment of clock-related diseases. Small-molecule compounds provide powerful tools to reveal novel functions of target proteins in the circadian clock mechanism, and can be great therapeutic candidates. Here we describe the detailed methods of measuring cellular circadian rhythms in a high-throughput manner for chemical screening to identify compounds that affect circadian rhythms by targeting clock-related proteins.
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