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Related Experiment Video

Updated: Sep 22, 2025

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

2.2K

Rodent Lung Tissue Sample Preparation and Processing for Shotgun Proteomics.

Hadeesha Piyadasa1,2,3, Ying Lao1, Oleg Krokhin1

  • 1Manitoba Centre for Proteomics and Systems Biology, Department of Internal Medicine, University of Manitoba, Winnipeg, MB, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|May 25, 2022
PubMed
Summary

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This study details a method for analyzing rodent lung proteins using liquid chromatography with tandem mass spectrometry (LC-MS/MS). This approach enables comprehensive protein profiling in lung tissue samples.

Area of Science:

  • Proteomics
  • Biochemistry
  • Analytical Chemistry

Background:

  • Mass spectrometry (MS) is a standard technique for global protein profiling.
  • Characterizing protein expression in biological samples is crucial for understanding biological processes.

Purpose of the Study:

  • To describe a detailed method for shotgun proteomics of rodent lung tissue.
  • To provide a reproducible protocol for sample preparation and LC-MS/MS analysis.

Main Methods:

  • Rodent lung tissue homogenization.
  • Sample preparation for mass spectrometry.
  • Liquid chromatography with tandem mass spectrometry (LC-MS/MS) analysis.

Main Results:

  • A comprehensive method for rodent lung tissue proteomics was established.
Keywords:
Mass spectrometryRodent lungsSample preparationShotgun proteomics

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Last Updated: Sep 22, 2025

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Comprehensive Workflow of Mass Spectrometry-based Shotgun Proteomics of Tissue Samples
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  • The protocol allows for detailed characterization of the lung proteome.

    • Conclusions:

    • The described LC-MS/MS method is suitable for in-depth proteomic analysis of rodent lung tissue.
    • This protocol can be applied to various studies involving lung biology and disease.