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Protein Purification and Digestion Methods for Bacterial Proteomic Analyses
Nicole Hansmeier1,2, Samrachana Sharma1, Tzu-Chiao Chao3,4
1Department of Biology, University of Regina, Regina, SK, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|May 25, 2022
Abstract:
Reproducible protein extraction is critical for the quantitative analysis of bacterial proteomes. While a wide range of techniques exist, there is no one-size-fits-all solution that will be suitable for all applications. In this report, we describe a set of standard extraction methods that have been adapted for a range of bacterial proteome analyses.
