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Updated: Sep 22, 2025
Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors
Published on: February 27, 2015
N-Terminomics/TAILS of Tissue and Liquid Biopsies
Anthonia Anowai1,2,3,4, Sameeksha Chopra1,2,3,4, Barbara Mainoli1,2,3,4
1Department of Physiology and Pharmacology, University of Calgary, Calgary, AB, Canada.
Abstract:
The N-terminomics approach of Terminal Amine Isotopic Labeling of Substrates (TAILS) enables the identification and quantification of natural and neo-N-termini of proteins using liquid chromatography and tandem mass spectrometry (LC-MS/MS). This methodology has been used to study protease function and identify protease substrates in cell culture systems, animal disease models, and more recently, has been applied to clinical samples. Here, we present the application of TAILS to tissue and liquid biopsies.
