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Related Concept Videos

Proteomics01:33

Proteomics

8.0K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Related Experiment Video

Updated: Sep 22, 2025

Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames
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Mass Spectrometry-Based Proteomics Analyses Using the OpenProt Database to Unveil Novel Proteins Translated from Non-Canonical Open Reading Frames

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Mining Proteomics Datasets to Uncover Functional Pseudogenes.

Anna Meller1, François-Michel Boisvert2

  • 1Department of Immunology and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Québec, QC, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|May 25, 2022
PubMed
Summary
This summary is machine-generated.

This study presents novel computational and experimental methods to identify and validate proteins encoded by pseudogenes. These techniques reveal previously undiscovered protein expression, enhancing our understanding of the human proteome.

Keywords:
Mass spectrometryParallel reaction monitoringProtein expressionProteomicsPseudogenes

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Area of Science:

  • Genomics
  • Proteomics
  • Bioinformatics

Background:

  • Gene annotation is complex, with challenges in identifying all protein-encoding genes.
  • The human genome sequence provides a foundation, but confirming protein expression requires further analysis.

Purpose of the Study:

  • To develop and validate methods for identifying pseudogene expression and the proteins they encode.
  • To enable comprehensive analysis of previously unsuspected protein expression.

Main Methods:

  • In silico analysis to identify evidence of pseudogene expression.
  • Experimental validation and quantification of pseudogene-encoded proteins.

Main Results:

  • Confirmed expression of proteins from pseudogenes, including alternative reading frames and isoforms.
  • Demonstrated a comprehensive approach to analyze gene expression into proteins.

Conclusions:

  • The developed techniques significantly improve gene annotation by revealing novel protein products.
  • This work expands the known human proteome and highlights the functional potential of pseudogenes.