Fluorine and nitrogen co-doped near-infrared carbon dots for fluorescence "on-off-on" determination of nitrite

A fluorescence "on-off-on" strategy was established for the determination of nitrite in aqueous solution based on fluorine and nitrogen co-doped near-infrared carbon dots (NIR-CDs). NIR-CDs were prepared via one-step hydrothermal method by using N-(4-aminophenyl)-acetamide and 4,5-difluorobenzene-1,2-diamine as precursors. The photoluminescence quantum yield of NIR-CDs reaches to 17.4%, and the optimal emission peak of NIR-CDs is 675 nm under excitation of 530 nm. The Stokes shift of NIR-CDs (145 nm) is higher than that of some CDs with longer emission wavelengths. The red bathophenanthroline disulfonic acid (BPS)-Fe²⁺ complex can quench the fluorescence of NIR-CDs via inner filter effect and static quench modes. Nitrite can oxidize Fe²⁺ to produce Fe³⁺ in acidic environment, resulting in not only the formation of colorless and unstable BPS-Fe³⁺ complex but also the fluorescence recovery of NIR-CDs. This fluorescence "on-off-on" phenomenon also comes with the color variation of the mixture, resulting in both the fluorescence and the visual determination of nitrite. Under optimal conditions, this assay exhibits a good linear range from 1 to 50 μM and a low detection limit of 0.056 μM for nitrite determination. The method showed good applicability for nitrite determination in soil extract, human urine, and water samples with acceptable results. A convenient fluorescence "on-off-on" strategy for nitrite detection based on fluorine and nitrogen co-doped near-infrared carbon dots (NIR-CDs) and bathophenanthroline disulfonic acid (BPS)-Fe²⁺ complex was innovatively established. This probe showed a low detection limit of 0.056 μM for nitrite in authentic samples, which offered a new sight for fluorescent and visual detection of nitrite in environmental protection and human health areas.