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Related Experiment Video

Updated: Sep 22, 2025

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives
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Vitrification does not affect birth weight: lessons from the oocyte donation model.

Joaquín Llácer1, Nerea Díaz1, Elisa Serrano-Revuelta2

  • 1Department of Reproductive Medicine, Instituto Bernabeu, Alicante, Spain.

Reproductive Biomedicine Online
|May 25, 2022
PubMed
Summary

Embryo cryopreservation using vitrification and warming does not increase the risk of high birth weight or large for gestational age (LGA) in singleton infants. This fertility treatment is safe for fetal growth outcomes.

Keywords:
Artificial cycleBirthweightFresh embryo transferLarge for gestational ageVitrified–warmed embryo transfer

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Area of Science:

  • Reproductive Medicine
  • Embryology
  • Neonatal Health

Background:

  • Embryo cryopreservation is a common technique in assisted reproductive technology.
  • Concerns exist regarding potential effects of vitrification and warming on fetal growth, specifically birth weight and gestational age.

Purpose of the Study:

  • To investigate whether embryo cryopreservation, specifically vitrification and warming, is associated with an increased incidence of high birth weight and large for gestational age (LGA) in singleton births.

Main Methods:

  • A retrospective cohort study included 670 oocyte recipients undergoing fresh or vitrified-warmed single blastocyst transfer.
  • Data were analyzed for maternal characteristics, pregnancy outcomes, and neonatal parameters, controlling for potential confounders.

Main Results:

  • No significant differences were observed in mean birth weight, macrosomia, or LGA between fresh and vitrified-warmed embryo transfer groups.
  • Key maternal and neonatal outcomes, including gestational age and pre-term birth, were comparable between the groups.

Conclusions:

  • Embryo vitrification and warming procedures do not adversely affect birth weight or increase the risk of LGA in singleton infants.
  • The study controlled for potential confounders, reinforcing the safety of cryopreservation techniques for fetal development.