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Updated: Sep 22, 2025

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
Published on: March 17, 2023
A hemicyanine-based near-infrared fluorescent probe for vapor-phase hydrazine detection and bioimaging in a complete
Yang Wang1, Xiao-Lei Xue2, Qi Zhang2
1Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China; College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Abstract:
A novel near-infrared fluorescent probe CyOE based on hemicyanine dye containing acetyl as a recognition site is reported. The probe CyOE shows high selectivity and sensitivity (LOD = 82 nM, 2.58 ppb), as well as good water solubility and quantitative detectability of hydrazine in the concentration range of 0-75 μM (R2 = 0.993). Moreover, CyOE has a significant increase in fluorescence at 735 nm with the addition of N2H4, which provides a rapid, colorimetric and gas-phase detection method for N2H4 in both aqueous solution and real water samples. In addition, CyOE is successfully utilized to visualize hydrazine in cells with low cytotoxicity and high cell permeability.

