Mutual effects of Shewanella putrefaciens-montmorillonite and their impact on uranium immobilization

This study investigated the immobilization behavior of U(VI) by the mixture of Shewanella putrefaciens (S. putrefaciens) and montmorillonite with batch experiment. The relevant mechanisms were discussed based on the experimental results and characterizations. It was found that the immobilization of U(VI) by S. putrefaciens-montmorillonite was inhibited at pH < 7.0 and enhanced at pH > 7.0. The inhibition effect was due to the aggregation and coverage between S. putrefaciens and montmorillonite, whereas the association of microbial dissolvable organic matters (DOM) on montmorillonite could promote immobilization of U(VI). The evidences of X-photoelectron spectroscopy (XPS) and density functional theory (DFT) simulation confirmed that the carboxyl-, hydroxyl-, nitrogen-based DOM do have the ability to interacted with U(VI). This work highlights a comprehensive and overlook perspective to understand the immobilization behavior of U(VI) in environmental organo-minerals.