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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Related Experiment Video

Updated: Sep 21, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Best practice standards for circular RNA research.

Anne F Nielsen^1,2, Albrecht Bindereif³, Irene Bozzoni⁴

¹Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark.

|May 26, 2022

Summary

This summary is machine-generated.

This perspective proposes essential experimental standards for circular RNA (circRNA) research. Implementing these best practices will advance the understanding of circRNA functions in eukaryotes.

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Last Updated: Sep 21, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Quantification of Circular RNAs Using Digital Droplet PCR

Quantification of Circular RNAs Using Digital Droplet PCR

Published on: September 16, 2022

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

Circular RNAs (circRNAs) are prevalent RNA molecules found across all life domains.
Recent years have seen a surge in circRNA research, highlighting the need for standardized methodologies.
The field of circRNA biology is relatively nascent, necessitating common experimental protocols.

Purpose of the Study:

To propose a set of guidelines and best practices for circRNA research.
To address the major class of eukaryotic circRNAs generated by spliceosome-mediated back-splicing.
To facilitate a deeper functional understanding of circRNAs.

Main Methods:

The authors draw upon their extensive experience in circRNA research.
The perspective focuses on spliceosome-catalyzed back-splicing events in Eukarya.
Guidelines cover isolation, analysis, expression, and depletion of circRNAs.

Main Results:

The proposed guidelines aim to establish common experimental standards.
These standards are crucial for the advancement of circRNA biology.
The perspective provides a framework for consistent circRNA research.

Conclusions:

Adoption of standardized experimental practices is critical for circRNA research.
Implementing these guidelines will accelerate the functional characterization of circRNAs.
This work aims to propel the field towards a more robust understanding of circRNA biology.