Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Systematic Mental Health Care for Airline Pilots: Addressing Rare but Catastrophic Safety Outcomes.

Asia-Pacific psychiatry : official journal of the Pacific Rim College of Psychiatrists·2026

Same author

Stress hyperglycemia ratio improves 28-day mortality prediction beyond GRACE score in critically ill patients with acute coronary syndrome: a retrospective cohort study from MIMIC-IV.

BMC cardiovascular disorders·2026

Same author

Corrigendum to "Analysis of the diagnostic value of pre-event intracardiac electrogram (pre-EGM) storage function in cardiac electronic implantable devices for arrhythmia diagnosis" [International Journal of Cardiology 442 (2026) 133881].

International journal of cardiology·2026

Same author

Higher Appendicular Skeletal Muscle Mass to Visceral Fat Area Ratio Associated With Reduced Risk of Prediabetes.

Diabetes, obesity & metabolism·2026

Same author

The synergistic effects of soy protein isolate and curdlan on salt-reduced hairtail sausages: Investigation into saltiness perception and quality characteristics.

Food chemistry: X·2026

Same author

Beyond birth rates: a theoretical framework for women's reproductive autonomy and quality childbearing.

Frontiers in public health·2026

Same journal

Long COVID and health-related quality of life: a systematic review of immune, inflammatory, and metabolic markers.

Frontiers in public health·2026

Same journal

Smart wearables for school-based physical activity promotion: K-12 physical education teachers' continuance intention and adoption pathways.

Frontiers in public health·2026

Same journal

Acceptance of an upper body exoskeleton for occupational tasks among soldiers.

Frontiers in public health·2026

Same journal

Effectiveness of a perforated spoon for reducing salt intake in a university cafeteria.

Frontiers in public health·2026

Same journal

Association of psychological resilience and social support with subjective coercion experience in hospitalized psychiatric inpatients: a cross-sectional study.

Frontiers in public health·2026

Same journal

The "5-4-3" model for weight management in psychiatric inpatients: a single-arm pre-post evaluation.

Frontiers in public health·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 21, 2025

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

Published on: June 23, 2022

Discourse on COVID-19 Mass Testing vs. Rapid Testing Processing

Ali Cheshmehzangi^1,2, Tong Zou²

¹Network for Education and Research on Peace and Sustainability (NERPS), Hiroshima University, Hiroshima, Japan.

Frontiers in Public Health

|May 27, 2022

Summary

No abstract available in PubMed .

Keywords:

COVID-19 containment mass testing rapid processing rapid testing test results

More Related Videos

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023

Related Experiment Videos

Last Updated: Sep 21, 2025

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling

Published on: June 23, 2022

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System

Published on: April 6, 2021

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Author Spotlight: Advancing Pathogen Diagnostics with Standardized LAMP

Published on: September 8, 2023