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Related Experiment Video

Updated: Sep 21, 2025

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

Contrasting behavior between the three human monocyte subsets in dengue pathophysiology.

Deepti Maheshwari¹, Keshav Saini¹, Prabhat Singh¹

¹ICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, India.

|May 27, 2022

Summary

Investigating monocyte subsets in dengue reveals distinct inflammatory roles. Intermediate monocytes drive inflammation and vascular issues in severe dengue, while nonclassical monocytes support vessel stability but decrease in circulation.

Keywords:

Immunology Omics Virology

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Last Updated: Sep 21, 2025

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis

Published on: October 17, 2018

Isolation, Transfection, and Culture of Primary Human Monocytes

Isolation, Transfection, and Culture of Primary Human Monocytes

Published on: December 16, 2019

Area of Science:

Immunology
Virology
Genetics

Background:

Monocytes are crucial in dengue pathophysiology.
Understanding monocyte subset-specific gene expression in vivo is limited.

Purpose of the Study:

To conduct a detailed transcriptional analysis of human monocyte subsets.
To compare gene expression in healthy children versus children with dengue febrile illness.

Main Methods:

Transcriptional analysis of three human monocyte subsets.
Comparison of gene expression profiles between healthy and dengue-affected children.

Main Results:

Intermediate monocytes (CD14+CD16high) from dengue patients showed upregulated genes for inflammation, endothelial dysfunction, and vascular permeability.
Classical monocytes (CD14+CD16low) shared some inflammatory features and increased in severe dengue.
Nonclassical monocytes (CD14-CD16high) upregulated genes for vasoconstriction and endothelial stability, declining in circulation.

Conclusions:

Distinct transcriptional profiles of monocyte subsets are associated with dengue severity.
Findings elucidate the specific roles of monocyte subsets in dengue pathogenesis.