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Correction: Komatsu et al. Three-Dimensional Visualization and Detection of the Pulmonary Venous-Left Atrium Connection Using Artificial Intelligence in Fetal Cardiac Ultrasound Screening. <i>Bioengineering</i> 2026, <i>13</i>, 100.

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Decellularization of the Murine Cardiopulmonary Complex
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An Optimized Method to Decellularize Human Trabecular Meshwork.

Devon J Crouch1, Carl M Sheridan1, Julia G Behnsen2

  • 1Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, Faculty of Health and Life Sciences, University of Liverpool, Liverpool L7 8TX, UK.

Bioengineering (Basel, Switzerland)
|May 27, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed a new method to remove cells from the trabecular meshwork (TM) while preserving its structure. This technique using ammonium hydroxide is crucial for creating biomimetic scaffolds for glaucoma research and tissue regeneration.

Keywords:
X-ray computed tomographyammonium hydroxidebioengineeringdecellularizationeyeglaucomalysing agenttrabecular meshworktrypsin

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Area of Science:

  • Biomaterials Science
  • Ophthalmology
  • Tissue Engineering

Background:

  • Glaucoma is associated with elevated intraocular pressure (IOP), often due to dysfunction in the trabecular meshwork (TM).
  • The TM's complex 3D structure is vital for regulating aqueous humor outflow and IOP.
  • Current therapeutic options for TM dysfunction are limited, necessitating advanced models and regenerative strategies.

Purpose of the Study:

  • To develop an optimized protocol for decellularizing the trabecular meshwork (TM) while preserving its intricate 3D structure.
  • To create biomimetic scaffolds for glaucoma research and potential TM tissue regeneration.
  • To evaluate the efficacy of different lysing agents for TM decellularization.

Main Methods:

  • Tested Trypsin and Ammonium Hydroxide (NH4OH) as lysing agents on bovine and human TM tissue.
  • Assessed cell removal using dsDNA quantification.
  • Evaluated structural integrity using light microscopy, Scanning Electron Microscopy (SEM), and X-ray computed tomography.

Main Results:

  • Ammonium Hydroxide (NH4OH) effectively removed cells from both bovine and human TM tissue (dsDNA levels significantly reduced).
  • NH4OH treatment preserved the structural integrity of the TM, unlike Trypsin which caused significant disruption.
  • X-ray computed tomography provided novel 3D reconstructions of decellularized human TM, revealing its complex architecture.

Conclusions:

  • A validated protocol using 2% NH4OH effectively decellularizes human TM without compromising its delicate structure.
  • This method provides a promising approach for generating biomimetic scaffolds for glaucoma research and TM tissue regeneration.
  • The preserved intricate architecture of decellularized TM is suitable for developing advanced in vitro models or implantable devices.