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Related Experiment Video

Updated: Sep 21, 2025

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy
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Self-Assembled Hydrogel Microparticle-Based Tooth-Germ Organoids.

Cemile Kilic Bektas1, Weibo Zhang2, Yong Mao1

  • 1Department of Chemistry and Chemical Biology, Rutgers University, 123 Bevier Rd, Piscataway, NJ 08854, USA.

Bioengineering (Basel, Switzerland)
|May 27, 2022
PubMed
Summary

Researchers developed novel tooth organoids using hydrogel microparticles (HMPs) seeded with stem cells. These self-assembling constructs show promise for future tooth regeneration strategies.

Keywords:
GelMA microparticleshuman dental pulp stem cells (hDPSCs)hydrogel microparticles (HMPs)porcine dental endothelial cells (pDE)self-assembled organoid structurestooth-bud organoids

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Area of Science:

  • Biomaterials Science
  • Developmental Biology
  • Regenerative Medicine

Background:

  • Creating functional tooth organoids for regeneration has been challenging.
  • Hydrogel microparticles (HMPs) offer scaffolding, cell delivery, and biomimicry for tissue engineering.

Purpose of the Study:

  • To investigate the use of methacrylated gelatin (GelMA)-derived HMPs for tooth organoid formation.
  • To characterize the self-assembly and cellular behavior within these novel tooth organoids.

Main Methods:

  • Human dental pulp stem cells (hDPSCs) and porcine dental epithelial cells (pDE) were seeded onto GelMA-HMPs.
  • Cell-seeded HMPs were cultured under conditions promoting self-assembly into organoids.
  • Organoid formation, cell viability, morphology, and differentiation were analyzed.

Main Results:

  • HMPs provided a suitable scaffold for hDPSC and pDE attachment and proliferation.
  • Self-assembled organoids reached ~50 mm³ within two weeks, maintaining cell viability and morphology.
  • Composite organoids exhibited polarized, differentiated dental cells, indicating tooth-bud-like development.

Conclusions:

  • GelMA-HMPs facilitate the self-assembly of dental cells into viable tooth-bud organoid-like structures.
  • This approach demonstrates potential for advancing tooth regeneration therapies.