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Related Experiment Video

Updated: Sep 21, 2025

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cfDNA-Based NGS IG Analysis in Lymphoma.

Christiane Pott1, Michaela Kotrova2, Nikos Darzentas2

  • 1Medical Department II, University Hospital Schleswig-Holstein, Kiel, Germany. c.pott@med2.uni-kiel.de.

Methods in Molecular Biology (Clifton, N.J.)
|May 27, 2022
PubMed
Summary

Liquid biopsy offers a noninvasive method for monitoring cancer. This study details a new assay for detecting minimal residual disease (MRD) in B-cell lymphomas using circulating tumor DNA (ctDNA) and immunoglobulin gene rearrangements.

Keywords:
Cell-free DNADigital droplet PCRImmunoglobulin rearrangementsLiquid biopsyMinimal residual diseaseNext-generation sequencingPlasmaTherapy monitoring

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Area of Science:

  • Oncology
  • Molecular Diagnostics
  • Genetics

Background:

  • Liquid biopsy analyzes body fluids for molecular tumor profiling.
  • Circulating tumor DNA (ctDNA) shows promise in lymphoma diagnostics and prognostics.
  • Clonal immunoglobulin (IG) gene rearrangements are specific biomarkers for lymphoid malignancies.

Purpose of the Study:

  • To develop a reproducible next-generation sequencing (NGS) assay.
  • To identify and characterize clonal IG gene rearrangements in cell-free DNA (cfDNA).
  • To enable minimal residual disease (MRD) detection in B-cell lymphomas.

Main Methods:

  • Utilized next-generation sequencing (NGS) technology.
  • Focused on analyzing cell-free DNA (cfDNA) for genetic alterations.
  • Targeted clonal immunoglobulin (IG) gene rearrangements as biomarkers.

Main Results:

  • Developed a reproducible NGS assay for IG gene rearrangement detection.
  • Demonstrated the feasibility of MRD assessment in B-cell lymphomas using ctDNA.
  • Established a method for characterizing specific molecular targets in cfDNA.

Conclusions:

  • The described NGS assay is suitable for MRD detection in B-cell lymphomas.
  • ctDNA analysis of IG gene rearrangements provides a noninvasive approach for monitoring treatment response.
  • This method advances the diagnostic and prognostic capabilities of liquid biopsy in hematologic malignancies.