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Updated: Jun 26, 2026

3' End Sequencing Library Preparation with A-seq2
12:01

3' End Sequencing Library Preparation with A-seq2

Published on: October 10, 2017

AIRR Community Guide to Planning and Performing AIRR-Seq Experiments

Anne Eugster1, Magnolia L Bostick2,3, Nidhi Gupta2

  • 1Center for Regenerative Therapies Dresden, Faculty of Medicine, TU Dresden, Dresden, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|May 27, 2022
PubMed

Keywords:
AIRR-seqAntibodyImmune repertoireImmunoglobulinNext-generation sequencingT-cell receptorV(D)J recombination

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...