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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
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ARResT/Interrogate Immunoprofiling Platform: Concepts, Workflows, and Insights.

Nikos Darzentas1

  • 1Department of Hematology, University of Schleswig-Holstein, Kiel, Germany. nikos.darzentas@uksh.de.

Methods in Molecular Biology (Clifton, N.J.)
|May 27, 2022
PubMed
Summary

The ARResT/Interrogate platform enables high-throughput sequencing for immunoglobulin (IG) and T-cell receptor (TR) repertoire profiling. This study details its concepts, workflow, and practical insights for researchers.

Keywords:
Antigen receptorsBioinformaticsIGImmunoglobulinPipelineSequence analysisTR

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Area of Science:

  • Immunology
  • Genomics
  • Bioinformatics

Background:

  • High-throughput sequencing is crucial for analyzing immunoglobulin (IG) and T-cell receptor (TR) repertoires.
  • Standardized and efficient assays are needed for reliable repertoire profiling.

Purpose of the Study:

  • To introduce the ARResT/Interrogate platform for IG and TR repertoire analysis.
  • To describe the platform's concepts, workflow, and EuroClonality-NGS specific features.
  • To share practical experiences and insights from using the ARResT/Interrogate platform.

Main Methods:

  • Development and application of high-throughput sequence-based assays.
  • Profiling of immunoglobulin (IG) and T-cell receptor (TR) repertoires.
  • Utilizing the ARResT/Interrogate platform and EuroClonality-NGS methodologies.

Main Results:

  • The ARResT/Interrogate platform provides a framework for IG and TR repertoire profiling.
  • The study outlines the platform's core concepts and workflow.
  • Experiences with the platform offer insights into its application.

Conclusions:

  • ARResT/Interrogate facilitates advanced IG and TR repertoire analysis.
  • The platform supports the EuroClonality-NGS initiative.
  • This work provides valuable guidance for researchers using high-throughput repertoire sequencing.