Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Validation of the Electrophilic Allergen Screening Assay for Detection of Key Event 1 of the Skin Sensitization Adverse Outcome Pathway.

Toxics·2026
Same author

A single-cell multi-omics assay for simultaneous measurement of vector copy number and protein expression in CAR T cells.

Molecular therapy. Advances·2026
Same author

Correction to "Potent, Selective Pyrrolopyrimidine PDE11A4 Inhibitors with Improved Pharmaceutical Properties".

ACS medicinal chemistry letters·2026
Same author

P2X7R-mediated IL-1β release by human brain tissue: the impact of CNS-penetrant potential therapeutics.

Brain : a journal of neurology·2026
Same author

Potent, Selective Pyrrolopyrimidine PDE11A4 Inhibitors with Improved Pharmaceutical Properties.

ACS medicinal chemistry letters·2026
Same author

Combinatorial chemistry identifies additional compounds that selectively inhibit DLK-dependent retrograde signaling while minimally affecting other axonal roles of DLK.

Scientific reports·2026

Related Experiment Video

Updated: Sep 21, 2025

Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

3.6K

Development of a 96-Well Electrophilic Allergen Screening Assay for Skin Sensitization Using a Measurement Science

Elijah J Petersen1, Richard Uhl2, Blaza Toman3

  • 1Biosystems and Biomaterials Division, Material Measurement Laboratory, National Institute of Standards and Technology (NIST), 100 Bureau Drive, Gaithersburg, MD 20899, USA.

Toxics
|May 27, 2022
PubMed
Summary

The Electrophilic Allergen Screening Assay (EASA) was improved using a 96-well plate format, enhancing skin sensitization testing. This optimized assay shows 77% concordance with animal data, offering a reliable alternative for chemical safety assessment.

Keywords:
adverse outcome pathwaydirect peptide reactivity assayin vitro methodmeasurement sciencemetrologynew approach methodologyskin sensitization

More Related Videos

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

16.5K
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
06:34

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

Published on: June 4, 2017

10.1K

Related Experiment Videos

Last Updated: Sep 21, 2025

Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

3.6K
Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

16.5K
A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma
06:34

A Component-resolved Diagnostic Approach for a Study on Grass Pollen Allergens in Chinese Southerners with Allergic Rhinitis and/or Asthma

Published on: June 4, 2017

10.1K

Area of Science:

  • Toxicology
  • Chemical Biology
  • Assay Development

Background:

  • Skin sensitization is a key adverse outcome pathway (AOP) event.
  • The Electrophilic Allergen Screening Assay (EASA) is an in chemico method for detecting skin sensitization.
  • Previous cuvette-based EASA had limitations in throughput and control measurements.

Purpose of the Study:

  • To redesign the EASA into a 96-well plate format.
  • To incorporate in-process controls to quantify assay variability.
  • To improve the reliability and throughput of in chemico skin sensitization testing.

Main Methods:

  • Redesigned EASA into a 96-well plate format.
  • Incorporated in-process control measurements to monitor variability.
  • Analyzed 67 test compounds (TCs) using the new format.

Main Results:

  • The 96-well EASA demonstrated 77% concordance with local lymph node assay (LLNA) animal data.
  • Performance was consistent with the OECD test guideline 442C (DPRA).
  • In-process controls helped quantify sources of variability.

Conclusions:

  • The 96-well plate EASA enhances confidence in in chemico skin sensitization assays.
  • Characterizing variability and using controls are crucial for assay development.
  • This improved EASA offers a more reliable and efficient method for skin sensitization testing.