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A peptide aptamer based electrochemical amperometric sensor for sensitive L-glutamate detection
Wenjing Wang1, Yumin He2, Yunling Gao3
1College of Chemistry and Materials Science, Hunan Agricultural University, Changsha, Hunan 410128, PR China; Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Engineering Research Canter for Healthy Livestock and Poultry Production, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan 410125, PR China.
This study presents a novel enzyme-free biosensor for detecting L-glutamate (L-Glu), crucial for umami taste and brain function. The peptide-based sensor on a gold electrode offers high sensitivity and specificity for L-Glu determination.
Area of Science:
- Electrochemistry
- Biosensor Technology
- Analytical Chemistry
Background:
- L-glutamate (L-Glu) is vital for the umami taste and central nervous system functions.
- Accurate detection of L-Glu is essential for various biological and food science applications.
- Existing detection methods may lack sensitivity or specificity.
Purpose of the Study:
- To design and fabricate a novel enzyme-free amperometric biosensor for specific L-Glu determination.
- To characterize the performance of the developed peptide-based biosensor.
- To evaluate the biosensor's applicability in complex biological samples.
Main Methods:
- Fabrication of an enzyme-free amperometric biosensor using a self-assembled peptide on a gold electrode.
- Characterization using cyclic voltammetry, electrochemical impedance spectroscopy, AFM, and SEM.
- Performance evaluation including sensitivity, detection limit, specificity, and linear range.
Main Results:
- The biosensor demonstrated optimal response at 0.10 V in phosphate-buffered saline within 200 s.
- Achieved high sensitivity with a low detection limit of 1.00 × 10-10 M.
- Exhibited excellent specificity, a wide linear range, and reliable performance in diluted mouse serum samples (RSD < 5.00%, recovery 93.32%-105.15%).
Conclusions:
- The developed peptide-based amperometric biosensor is a highly sensitive and specific tool for L-Glu detection.
- The enzyme-free approach offers a promising alternative for L-Glu monitoring.
- The biosensor shows potential for practical applications in analyzing biological samples.
