Related Experiment Video
Updated: Sep 21, 2025
08:14
MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening
Published on: October 26, 2017
15.8K
An Interfacial Affinity Interaction-Based Method for Detecting HOTAIR lncRNA in Cancer Plasma Samples
Kimberley Clack1,2, Narshone Soda2, Surasak Kasetsirikul2,3
1School of Environment and Science, Nathan Campus, Griffith University, Nathan, QLD 4111, Australia.
Biosensors
|May 28, 2022
Summary
We developed a novel electrochemical method to detect HOTAIR, a long non-coding RNA linked to ovarian cancer. This rapid assay accurately identifies HOTAIR in cell lines and patient samples, distinguishing cancerous from benign conditions.
Area of Science:
- Biochemistry
- Molecular Biology
- Biosensors
Background:
- Long non-coding RNA HOTAIR is implicated in ovarian cancer development and progression.
- Aberrant HOTAIR expression is a hallmark of various cancers, including ovarian cancer.
- Sensitive and specific detection of HOTAIR is crucial for early diagnosis and monitoring.
Purpose of the Study:
- To develop a novel, rapid, and simple electrochemical method for detecting HOTAIR.
- To quantify HOTAIR in ovarian cancer cell lines and patient plasma samples.
- To assess the specificity and analytical performance of the developed biosensor.
Main Methods:
- Extraction and magnetic bead-based purification of HOTAIR lncRNA from biological samples.
- Direct adsorption of purified HOTAIR onto screen-printed gold electrodes (SPE-Au).
- Electrochemical quantification using a [Fe(CN)6]3-/4- redox couple.
Main Results:
- The assay demonstrated a linear dynamic range from 1 pM to 100 nM for HOTAIR detection.
- High specificity was observed, distinguishing HOTAIR from non-specific microRNAs (miR-891, miR-486).
- The method successfully differentiated between ovarian cancer patient samples and benign samples.
Conclusions:
- A novel electrochemical biosensor for rapid and sensitive HOTAIR detection has been established.
- The developed method shows promise for clinical application in ovarian cancer diagnosis.
- This approach offers a valuable tool for studying the role of HOTAIR in cancer biology.
