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Updated: Sep 21, 2025
Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
Published on: February 16, 2018
Molecularly Imprinted Polymer-Amyloid Fibril-Based Electrochemical Biosensor for Ultrasensitive Detection of
Ibrar Alam1, Benchaporn Lertanantawong2, Thana Sutthibutpong3,4,5
1Nanoscience and Nanotechnology, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand.
Abstract:
A tryptophan (Trp) sensor was investigated based on electrochemical impedance spectroscopy (EIS) of a molecularly imprinted polymer on a lysozyme amyloid fibril (MIP-AF). The MIP-AF was composed of aniline as a monomer chemically polymerized in the presence of a Trp template molecule onto the AF surface. After extracting the template molecule, the MIP-AF had cavities with a high affinity for the Trp molecules. The obtained MIP-AF demonstrated rapid Trp adsorption and substantial binding capacity (50 µM mg-1). Trp determination was studied using non-Faradaic EIS by drop drying the MIP-AF on the working electrode of a screen-printed electrode. The MIP-AF provided a large linear range (10 pM-80 µM), a low detection limit (8 pM), and high selectivity for Trp determination. Furthermore, the proposed method also indicates that the MIP-AF can be used to determine Trp in real samples such as milk and cancer cell media.
