Analysis of Mn²⁺ and Zn²⁺ Ions in Macroalgae with Heteroelement-Doped Carbon-Based Fluorescent Probe

Kelp and laver are large economic macroalgae in China, which are rich in nutrients, especially Mn and Zn. Excessive intake of Mn and Zn can be harmful to the human body. Therefore, it is necessary to develop a convenient and efficient method to detect the contents of Mn and Zn in macroalgae. In this experiment, red carbon dots (R-CDs) doped with N and S elements were prepared by the thermal solvent method. The obtained R-CDs displayed excitation wavelength-independent fluorescent emission in the red spectral region. The R-CDs were used to construct a fluorescent probe for specific recognition of Mn²⁺ and Zn²⁺, achieving high-sensitivity detection of Mn²⁺ and Zn²⁺. The detection results showed a good linear relationship between fluorescence intensity and Mn²⁺ concentration, and the calculated detection limit was 0.23 nmol/L. For the detection of Zn²⁺, the detection limit was estimated as 19.1 nmol/L. At the same time, the content distribution of Mn and Zn elements in macroalgae produced in Fujian was investigated by the constructed fluorescence probe. It was found that kelp, laver, and their products are rich in Mn and Zn elements, and the content of Mn and Zn elements in laver is higher than that in kelp, which can be used as the optimal food supplement for Mn and Zn elements.