Platinum Nanoparticles: The Potential Antioxidant in the Human Lung Cancer Cells

Oxidative stress-related conditions associated with lung cells, specifically lung cancer, often lead to a poor prognosis. We hypothesized that platinum nanoparticles (PtNPs) can play a role in reversing oxidative stress in human lung adenocarcinoma A549 epithelial lung cell lines. Hydrogen peroxide (H₂O₂) was used to induce oxidative stress in cells, and the ability of PtNPs to lower the oxidative stress in the H₂O₂ treated epithelial lung cell line was determined. The differential capacity of PtNPs to remove H₂O₂ was studied through cell viability, nanoparticle uptake, DNA damage, ROS production, and antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). Results indicated that a higher concentration of PtNPs exhibited a higher antioxidant capacity and was able to reduce DNA damage and quench ROS production in the presence of 350 µM H₂O₂. All antioxidant enzymes' activities also increased in the PtNPs treatment. Our data suggested that PtNPs could be a promising antioxidant in the treatment of lung cancer.