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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Related Experiment Video

Updated: Sep 21, 2025

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes
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Nucleic Acid Aptamers in Nanotechnology.

Valentina V Sinitsyna1,2, Alexandre A Vetcher1,3

  • 1Nanotechnology Scientific and Educational Center, Institute of Biochemical Technology and Nanotechnology, Peoples' Friendship University of Russia (RUDN), Miklukho-Maklaya St. 6, Moscow 117198, Russia.

Biomedicines
|May 28, 2022
PubMed
Summary

Nucleic Acid (NA) aptamers, DNA and RNA oligonucleotides, offer unique structures for specific molecular recognition. Recent nanotechnological advances highlight their potential as macromolecular devices.

Keywords:
aptamer aptasensor(s)nanobiosensorsnanotubesnucleic acidsoligonucleotides

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Area of Science:

  • Biochemistry and Molecular Biology
  • Nanotechnology
  • Biotechnology

Background:

  • Nucleic Acid (NA) aptamers are short, single-stranded DNA or RNA molecules.
  • Their unique secondary and tertiary structures enable high affinity and specificity.
  • These structures arise from complementary base pairing and stacking interactions.

Purpose of the Study:

  • To review recent nanotechnological advances in the application of NA aptamers.
  • To highlight the potential of DNA and RNA aptamers as macromolecular devices.

Main Methods:

  • Review of existing literature on NA aptamers and nanotechnology.
  • Analysis of studies focusing on DNA and RNA aptamer applications.
  • Synthesis of information on structural properties and functional applications.

Main Results:

  • NA aptamers exhibit unique three-dimensional structures crucial for their function.
  • Significant progress has been made in applying aptamers in nanotechnological contexts.
  • DNA and RNA aptamers are versatile tools with broad application potential.

Conclusions:

  • NA aptamers are powerful tools due to their structural versatility and specificity.
  • Nanotechnology offers innovative approaches for aptamer development and application.
  • Further research in NA aptamer nanotechnology promises significant advancements.