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Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
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Visualizing 3D Embryo and Tissue Morphology-A Decade of Using High-Resolution Episcopic Microscopy (HREM) in

Stefan H Geyer1, Wolfgang J Weninger1

  • 1Division of Anatomy, Center for Anatomy and Cell Biology, Medical Imaging Cluster, Medical University of Vienna, Waehringer Str. 13, 1090 Vienna, Austria.

Biomedicines
|May 28, 2022
PubMed

Keywords:
volumetric reconstructionembryonic developmentmorphological analysisdigital histology

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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