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Related Experiment Video

Updated: Sep 21, 2025

On-Chip Endothelial Inflammatory Phenotyping
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Proinflammatory Endothelial Phenotype in Very Preterm Infants: A Pilot Study.

Giacomo S Amelio1, Livia Provitera1, Genny Raffaeli1,2

  • 1Neonatal Intensive Care Unit, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy.

Biomedicines
|May 28, 2022
PubMed
Summary

Very preterm infants exhibit a proinflammatory endothelial phenotype due to prenatal inflammation and early life stress. This phenotype, characterized by specific angiopoietin and adhesion molecule levels, may predict complications.

Keywords:
adhesion moleculesangiogenesisangiopoietinsendotheliuminfantinflammationnewborn

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Area of Science:

  • Neonatal Medicine
  • Vascular Biology
  • Perinatal Research

Background:

  • Very preterm infants (VPIs) face prenatal inflammation and postnatal complications.
  • Limited data exist on the endothelial effects of these conditions in VPIs.
  • Understanding the perinatal endothelial phenotype is crucial for predicting outcomes.

Purpose of the Study:

  • To investigate the perinatal endothelial phenotype in VPIs.
  • To explore the predictive value of this phenotype on neonatal mortality, hemodynamic, and respiratory complications.
  • To compare endothelial markers in VPIs with healthy full-term infants (FTIs).

Main Methods:

  • Pilot study involving 20 VPIs and 14 FTIs.
  • Measured concentrations of Angiopoietin-1 (Ang-1), Angiopoietin-2 (Ang-2), E-selectin, vascular adhesion molecule-1 (VCAM-1), tissue factor (TF), and endothelin-1 (ET-1).
  • Assays performed on days 1 (T1), 3 (T2), and 7-10 (T3) using Luminex technology.

Main Results:

  • VPIs showed lower Ang-1 and higher Ang-2/Ang-1 ratio at T1 and T2 compared to FTIs.
  • VPIs had lower E-selectin throughout and higher VCAM-1 at T1 and TF at T2.
  • Higher Ang-2 at T3 was observed in VPIs with hemodynamic or respiratory complications; no differences in markers were found in neonatal deaths.

Conclusions:

  • Perinatal low Ang-1 and high Ang-2, coupled with elevated VCAM-1 and TF, indicate a proinflammatory endothelial phenotype in VPIs.
  • This phenotype likely results from combined prenatal and postnatal stressors.
  • The findings suggest potential targets for intervention to mitigate complications in VPIs.