Related Experiment Video

Updated: Sep 21, 2025

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
14:04

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Published on: March 25, 2016

15.5K

Influence of Malignant Pleural Fluid from Lung Adenocarcinoma Patients on Neutrophil Response

Maria Mulet1, Rubén Osuna-Gómez1, Carlos Zamora1

  • 1Inflammatory Diseases, Institut de Recerca de l'Hospital de la Santa Creu i Sant Pau, Biomedical Research Institute Sant Pau (IIB Sant Pau), 08041 Barcelona, Spain.

Cancers
|May 28, 2022
PubMed

Insights

Malignant pleural effusions in lung adenocarcinoma promote neutrophil extracellular trap (NET) formation, which is linked to worse patient outcomes. Targeting NETs may offer a new therapeutic strategy for advanced lung cancer.

Area of Science:

  • Oncology
  • Immunology
  • Pulmonology

Background:

  • Malignant pleural effusion (MPE) is a severe complication of advanced lung adenocarcinoma (LAC).
  • Neutrophils in tumor infiltrates can drive tumor progression.
  • Elevated neutrophil counts in MPE correlate with poor prognosis in LAC.

Purpose of the Study:

  • To investigate phenotypical and functional alterations in neutrophils exposed to MPE.
  • To assess the impact of MPE immunomodulatory factors on neutrophil responses.
  • To explore associations between neutrophil functions and clinical outcomes in LAC patients.

Main Methods:

  • Collected pleural fluid from 47 LAC patients and 25 heart failure (HF) patients.
  • Quantified neutrophil degranulation products using ELISA.
  • Assessed neutrophil oxidative burst, apoptosis, and NETosis via flow cytometry and fluorescence microscopy.

Main Results:

  • Neutrophils in MPE-LAC showed increased survival and neutrophil extracellular trap (NET) formation compared to PE-HF.
  • Oxidative burst capacity was reduced in neutrophils cultured with MPE-LAC.
  • NETosis positively correlated with MMP-9, P-selectin, and sPD-L1, and was associated with worse clinical outcomes.

Conclusions:

  • This study establishes a link between NETosis and poor prognosis in MPE-associated LAC.
  • Neutrophils, via NET release, may actively contribute to tumor progression in MPE.
  • NETs represent a potential therapeutic target for advanced lung adenocarcinoma.
Keywords:
NETosislung adenocarcinomamalignant pleural fluidneutrophils

More Related Videos

Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment08:59

Neutrophil Extracellular Traps Generated by Low Density Neutrophils Obtained from Peritoneal Lavage Fluid Mediate Tumor Cell Growth and Attachment

Published on: August 3, 2018

8.9K
A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide08:33

A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide

Published on: December 15, 2014

11.9K

Related Concept Videos

Pleural Effusion II: Symptoms and Management01:28

Pleural Effusion II: Symptoms and Management

Pleural Effusion Overview
A pleural effusion is the abnormal collection of fluid between the parietal and visceral pleura layers of tissue that form the lining of the lungs and chest cavity. It can occur independently or due to surrounding parenchymal diseases, such as infection, malignancy, or inflammatory conditions.
Clinical Manifestations:
279
Pleural Effusion I: Introduction01:25

Pleural Effusion I: Introduction

Pleural effusion is an abnormal fluid accumulation in the pleural cavity, a narrow space between the lungs and the chest wall. It is not a disease per se but rather a symptom or indication of an underlying disease. In normal circumstances, this space contains a small amount of fluid (5 to 15 mL), a lubricant facilitating the non-frictional movement of the pleural surfaces.
There are two main types of pleural effusion: transudative and exudative. They are differentiated using Light's...
2.0K