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Updated: Sep 21, 2025
Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
Published on: October 12, 2018
Single-Cell and Single-Nucleus RNAseq Analysis of Adult Neurogenesis
Alena Kalinina1, Diane Lagace1
1Neuroscience Program, Department of Cellular and Molecular Medicine, Ottawa Hospital Research Institute, Brain and Mind Research Institute, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
Abstract:
The complexity of adult neurogenesis is becoming increasingly apparent as we learn more about cellular heterogeneity and diversity of the neurogenic lineages and stem cell niches within the adult brain. This complexity has been unraveled in part due to single-cell and single-nucleus RNA sequencing (sc-RNAseq and sn-RNAseq) studies that have focused on adult neurogenesis. This review summarizes 33 published studies in the field of adult neurogenesis that have used sc- or sn-RNAseq methods to answer questions about the three main regions that host adult neural stem cells (NSCs): the subventricular zone (SVZ), the dentate gyrus (DG) of the hippocampus, and the hypothalamus. The review explores the similarities and differences in methodology between these studies and provides an overview of how these studies have advanced the field and expanded possibilities for the future.
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