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Related Experiment Video

Updated: Sep 21, 2025

Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
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Recombinant Mitochondrial Genomes Reveal Recent Interspecific Hybridization between Invasive Salangid Fishes.

Evgeniy S Balakirev1

  • 1A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok 690041, Russia.

Life (Basel, Switzerland)
|May 28, 2022
PubMed
Summary

Interspecific recombination in mitochondrial DNA of Protosalanx chinensis suggests recent hybridization with Neosalanx species, driven by human activities. These findings offer genetic markers to manage invasive hybrid salangid fishes.

Keywords:
Salangidaeclearhead icefish Protosalanx chinensisinvasive species hybridizationmitochondrial genomerecombinationshort-snout icefish Neosalanx tangkahkeii

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Area of Science:

  • Genetics
  • Evolutionary Biology
  • Aquaculture Science

Background:

  • Human activities like translocation and habitat modification can break reproductive barriers, leading to interspecific mitochondrial genome recombination.
  • The potential for hybridization and mitochondrial DNA (mtDNA) recombination in commercially important and invasive salangid fishes, such as Protosalanx chinensis, remains understudied.
  • Understanding hybridization events is crucial for managing invasive species and their ecological impact.

Purpose of the Study:

  • To investigate the patterns of diversity and recombination in the mitochondrial genomes of Protosalanx chinensis and related salangid species.
  • To assess the probability of interspecific hybridization as a cause for observed genetic patterns in P. chinensis.
  • To identify genetic markers for distinguishing hybrid salangid fishes and controlling their invasive spread.

Main Methods:

  • Analysis of complete mitochondrial (mt) genomes from GenBank resources for P. chinensis, Neosalanx tangkahkeii, and N. anderssoni.
  • Sliding window analysis to identify regions of high divergence within the P. chinensis mt genome.
  • Pairwise homoplasy index (PHI) test to detect significant signals of recombination.

Main Results:

  • Four distinct regions of high divergence were identified in the P. chinensis mt genome, correlating with specific genes (COI, ND4L-ND4, ND5) and the control region.
  • These divergent regions showed high sequence similarity to the mt genomes of N. tangkahkeii and N. anderssoni, suggesting introgressed fragments.
  • Four significant signals of recombination were detected, indicating recent interspecific hybridization between P. chinensis and the Neosalanx species. The recombinant fragments were not fixed, resulting in mosaic mt genomes.
  • Alternative explanations like taxonomic errors or contamination were ruled out by the data.

Conclusions:

  • The observed genetic patterns in P. chinensis are strong evidence of recent interspecific hybridization with Neosalanx species.
  • The identified recombinant fragments serve as diagnostic genetic markers for hybrid identification.
  • These markers can be instrumental in monitoring and controlling the invasive dynamics of hybrid salangid fishes.