Related Experiment Video
Updated: Sep 21, 2025
Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
Circulating miRNA-195-5p and -451a in Patients with Acute Hemorrhagic Stroke in Emergency Department
Mauro Giordano1,2, Maria Consiglia Trotta3, Tiziana Ciarambino4
1Department of Advanced Medical and Surgical Sciences, University of Campania "Luigi Vanvitelli", 80138 Naples, Italy.
Abstract:
(1) Background: In our previous study, acute ischemic stroke (AIS) patients showed increased levels of circulating miRNAs (-195-5p and -451a) involved in vascular endothelial growth factor A (VEGF-A) regulation. Here, we evaluated, for the first time, both circulating miRNAs in acute intracerebral hemorrhagic (ICH) patients. (2) Methods: Circulating miRNAs and serum VEGF-A were assessed by real-time PCR and ELISA in 20 acute ICH, 21 AIS patients, and 21 controls. These were evaluated at hospital admission (T0) and after 96 h (T96) from admission. (3) Results: At T0, circulating miRNAs were five-times up-regulated in AIS patients, tending to decrease at T96. By contrast, in the acute ICH group, circulating miRNAs were significantly increased at both T0 and T96. Moreover, a significant decrease was observed in serum VEGF-A levels at T0 in AIS patients, tending to increase at T96. Conversely, in acute ICH patients, the levels of VEGF-A were significantly decreased at both T0 and T96. (4) Conclusions: The absence of a reduction in circulating miRNAs (195-5p and -451a), reported in acute ICH subjects after 96 h from hospital admission, together with the absence of increment of serum VEGF-A, may represent useful biomarkers indicating the severe brain damage status that characterizes acute ICH patients.
Insights
In acute intracerebral hemorrhage (ICH) patients, circulating miRNAs (195-5p and -451a) remained elevated, unlike in acute ischemic stroke (AIS). This, with decreased VEGF-A, may indicate severe brain damage in ICH.
Area of Science:
- Neuroscience
- Biochemistry
- Genetics
Background:
- Previous research identified elevated circulating microRNAs (miRNAs) -195-5p and -451a in acute ischemic stroke (AIS) patients, linked to vascular endothelial growth factor A (VEGF-A) regulation.
- This study investigates these miRNAs in acute intracerebral hemorrhage (ICH) patients for the first time.
Purpose of the Study:
- To compare circulating miRNA and VEGF-A levels in acute ICH patients versus AIS patients and controls.
- To assess changes in these biomarkers over 96 hours from hospital admission.
Main Methods:
- Real-time PCR and ELISA were used to measure circulating miRNAs and serum VEGF-A.
- Samples were collected from 20 acute ICH patients, 21 AIS patients, and 21 controls at admission (T0) and 96 hours later (T96).
Main Results:
- In AIS patients, circulating miRNAs were upregulated at T0 and tended to decrease at T96, while VEGF-A decreased at T0 and tended to increase at T96.
- In contrast, acute ICH patients showed significantly increased circulating miRNAs at both T0 and T96.
- Acute ICH patients exhibited significantly decreased VEGF-A levels at both T0 and T96.
Conclusions:
- Sustained high levels of circulating miRNAs (-195-5p and -451a) and low VEGF-A in acute ICH patients after 96 hours may indicate severe brain damage.
- These biomarkers could potentially serve as indicators of the severity of brain damage in acute ICH.
