Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Sep 21, 2025

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology
07:03

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Published on: December 1, 2023

1.0K

Reversible Thermo-Responsive Valve for Microfluidic Paper-Based Analytical Devices.

Hiroki Toda1,2, Wataru Iwasaki2, Nobutomo Morita2

  • 1Graduate School of Science and Technology, Kumamoto University, Kumamoto 860-8555, Japan.

Micromachines
|May 28, 2022
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic Pitfalls of Action-Induced Tremor: A Case Report of Benign Adult Familial Myoclonic Epilepsy Treated with Thalamic Lesioning.

Movement disorders clinical practice·2026
Same author

Dual-readout villus-like scaffold microfluidic platform for quantitative analysis of gut microbiota formation under perfusion.

The Analyst·2026
Same author

IV Fosphenytoin for Acute Trigeminal Neuralgia: A Multicenter, Randomized, Double-Blind, Placebo-Controlled Trial.

Neurology·2026
Same author

Comparison of neuropsychological side effects between contemporary radiofrequency ablative neurosurgery for psychiatric disorders and conventional neurosurgical procedures: systematic review and meta-analysis.

Journal of neurology, neurosurgery, and psychiatry·2026
Same author

Lethally aggressive multiply recurrent adamantinomatous craniopharyngioma without histological malignancy: illustrative cases.

Journal of neurosurgery. Case lessons·2026
Same author

Long-Term Stable Biosensing Using Multiscale Biostructure-Preserving Metal Thin Films.

Biosensors·2026
This summary is machine-generated.

Researchers developed a novel thermo-responsive valve for microfluidic paper-based analytical devices (µPADs). This valve uses plasma-induced graft polymerization to control fluid flow, enabling applications like enzyme-linked immunosorbent assay (ELISA).

Area of Science:

  • Materials Science and Engineering
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Precise fluid control is essential for multi-reagent assays on microfluidic paper-based analytical devices (µPADs).
  • Existing methods for fluid control in µPADs often lack reusability or require complex external components.

Purpose of the Study:

  • To fabricate and characterize a novel thermo-responsive valve for microfluidic paper-based analytical devices (µPADs).
  • To demonstrate the valve's ability to control fluid flow based on temperature changes for potential application in enzyme-linked immunosorbent assay (ELISA).

Main Methods:

  • Fabrication of a thermo-responsive valve by polymerizing N-isopropylacrylamide onto a PVDF porous membrane using plasma-induced graft polymerization.
  • Characterization of pore structure changes using scanning electron microscopy (SEM).
Keywords:
microfluidic paper-based analytical devicesplasma-induced graft polymerizationpoly(N-isopropylacrylamide)thermo-responsive polymervalve

More Related Videos

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

21.3K
Thermal Measurement Techniques in Analytical Microfluidic Devices
08:29

Thermal Measurement Techniques in Analytical Microfluidic Devices

Published on: June 3, 2015

9.7K

Related Experiment Videos

Last Updated: Sep 21, 2025

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology
07:03

Author Spotlight: Integrating Computational and Experimental Approaches in Precision Oncology

Published on: December 1, 2023

1.0K
Microfluidic Chips Controlled with Elastomeric Microvalve Arrays
18:11

Microfluidic Chips Controlled with Elastomeric Microvalve Arrays

Published on: October 1, 2007

21.3K
Thermal Measurement Techniques in Analytical Microfluidic Devices
08:29

Thermal Measurement Techniques in Analytical Microfluidic Devices

Published on: June 3, 2015

9.7K
  • Evaluation of valve permeability to water and proteins at varying temperatures (below and above 32 °C).

    • Main Results:

    • The polymerized membrane exhibited reversible pore closure below 32 °C and opening above 32 °C.
    • The valve demonstrated impermeability to water and proteins below 32 °C and permeability to water above 32 °C.
    • The valve showed reliable, repeatable opening and closing by temperature cycling near 32 °C without performance degradation.

    Conclusions:

    • Plasma-induced graft polymerization is a viable method for creating high-performance thermo-responsive valves for µPADs.
    • The developed valve offers a promising solution for precise fluid control in µPADs, potentially enabling advanced applications like ELISA.