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Related Experiment Video

Updated: Sep 21, 2025

Author Spotlight: Advancing Intestinal Bacteria Cultivation for Poultry
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Quercetin Reduces Inflammation and Protects Gut Microbiota in Broilers.

Lei Sun1, Lewei Guo1, Gaoqing Xu1

  • 1College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.

Molecules (Basel, Switzerland)
|May 28, 2022
PubMed
Summary

Quercetin supplementation in broiler chickens combats inflammation and harmful gut bacteria caused by lipopolysaccharide (LPS) challenge. This dietary intervention helps restore beneficial intestinal microflora and protects jejunal integrity.

Keywords:
LPSbroiler chickeninflammatory responseintestinal microfloraquercetin

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Area of Science:

  • Poultry Science
  • Immunology
  • Microbiology

Background:

  • Lipopolysaccharide (LPS) induces significant inflammatory responses and gut dysbiosis in broiler chickens.
  • Intestinal barrier integrity is compromised by LPS, affecting tight junction proteins and apoptosis markers.
  • Alterations in jejunal microflora composition are observed following LPS challenge.

Purpose of the Study:

  • To investigate the protective effects of dietary quercetin against LPS-induced inflammation and microflora imbalance in broiler jejuna.
  • To evaluate quercetin's impact on inflammatory markers, intestinal barrier function, and microbial community structure.

Main Methods:

  • Broiler chickens were challenged with LPS and treated with quercetin (200 mg/kg).
  • Gene expression of inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8, IFN-γ), TLR-4, apoptosis (Bax, Caspase-3), and anti-apoptosis (Bcl-2) markers were analyzed.
  • Diamine oxidase (DAO) activity and tight junction proteins (ZO-1, Occludin) were measured.
  • Jejunal microflora composition was assessed at phylum and genus levels using sequencing.

Main Results:

  • LPS challenge increased pro-inflammatory markers and DAO activity while decreasing anti-apoptotic and tight junction protein expression.
  • Quercetin supplementation counteracted these LPS-induced negative effects, preserving intestinal barrier function.
  • LPS significantly reduced beneficial bacteria (e.g., Actinobacteria, Armatimonadetes) and specific genera, which quercetin restored.

Conclusions:

  • Dietary quercetin effectively alleviates LPS-induced inflammatory responses in broiler chickens.
  • Quercetin modulates jejunal microflora, restoring the balance of beneficial bacteria.
  • Quercetin represents a promising dietary strategy for improving gut health and immunity in poultry.