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Updated: Jun 19, 2026

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors

Published on: February 10, 2014

Pedestal High-Contrast Gratings for Biosensing.

Leonid Yu Beliaev¹, Peter Groth Stounbjerg², Giovanni Finco^1,3

¹DTU Fotonik-Department of Photonics Engineering, Technical University of Denmark, Ørsteds Plads, Building 345A, DK-2800 Kongens Lyngby, Denmark.

Nanomaterials (Basel, Switzerland)

|May 28, 2022

Summary

This summary is machine-generated.

Pedestal high-contrast gratings (PHCG) offer enhanced label-free biosensing performance. These PHCGs demonstrate superior sensitivity and lower detection limits for biomarkers compared to conventional HCGs.

Keywords:

atomic layer deposition avidin biosensing biotin bulk refractive index sensitivity high-contrast grating nanofabrication refractometric sensing silicon nanostructures surface sensitivity

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Area of Science:

Photonics
Nanotechnology
Biomedical Engineering

Background:

High-contrast gratings (HCG) are effective for label-free biomarker detection due to sharp optical resonances.
Conventional HCG designs have limitations in sensitivity and detection capabilities.

Purpose of the Study:

To experimentally evaluate the performance of pedestal HCG (PHCG) for biosensing.
To compare the sensitivity and detection limits of PHCG with conventional HCG.

Main Methods:

Fabrication and characterization of pedestal HCG (PHCG) and conventional HCG.
Measurement of bulk refractive index sensitivity and surface sensitivity using model analyte layers (Al2O3, HfO2, TiO2).
Avidin-biotin binding assay in solution to determine limit of detection (LoD) and limit of quantification (LoQ).

Main Results:

PHCG exhibited an 11.2% improvement in bulk refractive index sensitivity (536 nm/RIU vs. 482 nm/RIU).
PHCG showed a 10.5% improvement in surface sensitivity.
PHCG achieved a lower limit of detection (LoD) of 2.1 ng/mL and limit of quantification (LoQ) of 85 ng/mL for avidin detection, compared to conventional HCG (LoD: 3.2 ng/mL, LoQ: 213 ng/mL).

Conclusions:

Pedestal HCG demonstrates significantly enhanced performance for label-free biosensing.
The improved sensitivity and lower detection limits of PHCG make it highly suitable for detecting low analyte concentrations.
PHCG holds great potential for advanced biosensing applications.