Related Experiment Video

Updated: Sep 21, 2025

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
10:40

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods

Published on: December 21, 2019

26.1K

Identification and Functional Analyses of Host Proteins Interacting with the p17 Protein of Avian Reovirus

Chengcheng Zhang1,2,3, Xinyi Liu1,2,3, Fuxi Zhao1,2,3

  • 1College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China.

Viruses
|May 28, 2022
PubMed

Insights

Avian reovirus (ARV) p17 protein interacts with host factors like IGF2BP1 and PQBP1. IGF2BP1 promotes ARV replication, while PQBP1 inhibits it, offering insights into viral pathogenesis.

Area of Science:

  • Virology
  • Molecular Biology
  • Host-Pathogen Interactions

Background:

  • Avian reovirus (ARV) causes significant economic losses in poultry through diseases like viral arthritis and respiratory issues.
  • The precise molecular mechanisms underlying ARV pathogenesis, particularly the role of its proteins, remain incompletely understood.

Purpose of the Study:

  • To identify host cellular proteins interacting with the ARV p17 protein.
  • To elucidate the functional roles of these interacting proteins in ARV replication and pathogenesis.

Main Methods:

  • Yeast two-hybrid screening to identify ARV p17 interacting proteins from a chicken cDNA library.
  • Bioinformatic analyses (BLAST, Gene Ontology, KEGG) to classify identified proteins.
  • Subcellular localization studies using fluorescent protein tagging (GFP, RED).
  • Co-immunoprecipitation and GST pulldown assays to confirm direct interactions.
  • RT-qPCR to analyze gene expression changes in ARV-infected cells.
  • Functional assays to determine the effect of host proteins on viral replication.

Main Results:

  • Nineteen host proteins interacting with ARV p17 were identified, involved in multicellular organismal, metabolic, and regulatory processes.
  • ARV p17 colocalized with IGF2BP1 and PQBP1 in transfected cells; direct interactions were confirmed.
  • ARV infection altered the expression of several host genes, with IGF2BP1 upregulated and PQBP1 downregulated.
  • IGF2BP1 positively influenced ARV replication, whereas PQBP1 exhibited an inhibitory effect.

Conclusions:

  • The ARV p17 protein interacts with specific host factors, including IGF2BP1 and PQBP1.
  • These interactions play crucial roles in modulating ARV replication and pathogenesis.
  • Understanding these host-pathogen interactions provides valuable insights into ARV disease mechanisms.
Keywords:
avian reovirusp17 proteinprotein interactionyeast two hybrid

More Related Videos

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors06:02

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

7.0K
Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry09:38

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

8.2K

Related Concept Videos

Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.3K