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Molecular Analysis of Caprine Enterovirus Circulating in China during 2016-2021: Evolutionary Significance
Xiaoran Chang1, Qian Lin1, Qun Zhang1
1State Key Laboratory for Zoonotic Diseases, Key Laboratory of Zoonosis Research of Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun 130012, China.
Abstract:
Here, we report the characterization of 13 novel caprine/ovine enterovirus strains isolated from different regions in China during 2016-2021. Immunoperoxidase monolayer assay showed that these viral strains shared strong cross-reaction with the previously reported caprine enterovirus CEV-JL14. Alignment analysis of the complete nucleotide sequences revealed 79.2%-87.8% and 75.0%-76.7% sequence identity of these novel caprine enterovirus strains to CEV-JL14 and TB4-OEV, respectively. Phylogenetic analyses clustered these novel strains to EV-G based on the amino acid sequences of P1 and 2C+3CD. Moreover, phylogenetic analysis of these caprine enterovirus strains identified three new EV-G types using VP1 sequences. These results demonstrate the genetic variations and the evolution of caprine enterovirus.
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