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Related Experiment Video

Updated: Sep 21, 2025

Preparation of DNA-crosslinked Polyacrylamide Hydrogels
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Bulk pH-Responsive DNA Quadruplex Hydrogels Prepared by Liquid-Phase, Large-Scale DNA Synthesis.

Shizuma Tanaka, Shinsuke Yukami, Kazuki Fukushima

    ACS Macro Letters
    |May 28, 2022
    PubMed
    Summary

    Researchers developed a novel pH-responsive hydrogel using biocompatible oligodeoxynucleotides (ODN) and polyethylene glycol (PEG). This biomaterial forms stable hydrogels triggered by DNA i-motif formation, offering enhanced properties for potential applications.

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    Area of Science:

    • Biomaterials Science
    • Polymer Chemistry
    • Supramolecular Chemistry

    Background:

    • Development of advanced hydrogels with tunable properties is crucial for biomedical applications.
    • Biocompatible materials like oligodeoxynucleotides (ODN) and polyethylene glycol (PEG) are widely used in drug delivery and tissue engineering.
    • pH-responsive materials are desirable for controlled release applications.

    Purpose of the Study:

    • To synthesize and characterize a novel pH-responsive hydrogel biomaterial.
    • To explore the potential of DNA quadruplexes (i-motifs) as triggers for hydrogel formation and stability.
    • To investigate the impact of conjugate structure on hydrogel properties.

    Main Methods:

    • Synthesis of dC5-PEG conjugates via liquid-phase DNA synthesis and phosphoramidite chemistry.

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  • Preparation of hydrogels using dC5-PEG conjugates as macromonomers.
  • Characterization of hydrogel properties, including stability, stiffness, and rheology.
  • Investigation of i-motif formation as a trigger for hydrogelation.

    • Main Results:

    • Successfully prepared stable and stiff pH-responsive hydrogels from dC5-PEG conjugates.
    • Demonstrated that hydrogel formation and stability are triggered by DNA i-motif quadruplex formation.
    • Observed significantly enhanced thermal stabilities and nonlinearly improved rheological properties due to crosslinking and topological entanglement.

    Conclusions:

    • A novel, biocompatible, pH-responsive hydrogel was developed using ODN and PEG.
    • The DNA i-motif structure effectively triggers hydrogel formation and enhances material properties.
    • The findings suggest potential applications in areas requiring controlled material responses to pH changes.