Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

4.4K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
4.4K
EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

2.9K
Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
2.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exposure matters: The impact of exposure characteristics on In Vitro neurotoxicity of pesticides and implications for regulatory testing.

Toxicology·2026
Same author

Probing the Biological Plausibility of Fluoride as an Endocrine Disruptor.

Birth defects research·2026
Same author

Validation of the Epi2SensA Method Using the EpiDerm™ Model for Skin Sensitization Testing Under OECD TG442D.

Toxics·2026
Same author

A pipeline for developing AI-driven models to predict molecular initiating events: a case study on neural tube defects.

Journal of cheminformatics·2026
Same author

Commentary on GRADE Guidance 40: GRADE evidence-to-decision framework for environmental and occupational health.

Journal of clinical epidemiology·2026
Same author

Corrigendum to "Hypothesis-driven approach to developmental toxicity assessment: Using mechanistic information to inform testing" [Reprod. Toxicol. 140 (2026) 109119].

Reproductive toxicology (Elmsford, N.Y.)·2025

Related Experiment Video

Updated: Sep 21, 2025

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

12.7K

Pluripotent stem cell assays: Modalities and applications for predictive developmental toxicity.

Aldert H Piersma1, Nancy C Baker2, George P Daston3

  • 1Center for Health Protection, National Institute for Public Health and the Environment (RIVM), Bilthoven, The Netherlands.

Current Research in Toxicology
|May 31, 2022
PubMed
Summary

Embryonic stem cell-based models offer promising alternatives for developmental toxicity testing. This review details their capabilities and limitations, guiding future implementation in toxicology.

Keywords:
Developmental toxicityEmbryonic stem cell testPredictive toxicologyTeratogenesis

More Related Videos

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
09:02

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Published on: June 9, 2017

23.5K
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

9.4K

Related Experiment Videos

Last Updated: Sep 21, 2025

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
17:28

Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation

Published on: June 17, 2015

12.7K
Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
09:02

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Published on: June 9, 2017

23.5K
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

9.4K

Area of Science:

  • Toxicology
  • Developmental Biology
  • Stem Cell Research

Background:

  • Traditional animal testing for developmental toxicity faces ethical and practical challenges.
  • The field of developmental toxicology requires robust, validated alternative methods.
  • Pluripotent stem cells provide a powerful platform for in vitro developmental toxicity assays.

Purpose of the Study:

  • To review the diversity and evolution of embryonic stem cell-based models for developmental toxicity testing.
  • To critically evaluate the applicability and limitations of these alternative assays.
  • To identify pathways for regulatory implementation of novel toxicology methods.

Main Methods:

  • A systematic scoping review was conducted following a published protocol.
  • Inclusion criteria were applied to select relevant stem cell-based assays.
  • Assays were critically assessed based on biological domain, endpoints, standardization, chemical domain, reproducibility, and predictive power.

Main Results:

  • A wide array of embryonic stem cell-based assays have been developed over the past 50 years.
  • Detailed analysis reveals variability in assay design, endpoints, and validation status.
  • Key challenges include standardization, reproducibility, and demonstrating predictive power for regulatory use.

Conclusions:

  • Embryonic stem cell-based models hold significant potential as alternatives in developmental toxicity testing.
  • Further research and standardization are crucial for their successful regulatory implementation.
  • This review provides a roadmap for advancing these alternative methods in toxicology.