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Updated: Sep 21, 2025

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
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IRimage: open source software for processing images from infrared thermal cameras.

Gustavo Pereyra Irujo1

  • 1Instituto Nacional de Tecnología Agropecuaria (INTA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Balcarce, Buenos Aires, Argentina.

Peerj. Computer Science
|May 31, 2022
PubMed
Summary

IRimage software enhances thermal image analysis for affordable cameras, improving data accuracy and reproducibility. This tool aids researchers in obtaining reliable temperature measurements for scientific reporting and analysis.

Keywords:
Consumer thermal camerasFIJIImage processingImageJInfraredOpen sourceReproducibilityTemperatureThermalThermography

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Area of Science:

  • Thermal imaging
  • Image analysis
  • Scientific instrumentation

Background:

  • Affordable consumer-grade thermal cameras offer potential for research but often lack robust data processing.
  • Existing thermal imaging software may not meet the demands for high throughput, accuracy, and reproducibility in scientific studies.

Purpose of the Study:

  • To develop and validate IRimage, an open-source software tool for processing thermal images.
  • To enhance the throughput, accuracy, and reproducibility of temperature measurements from consumer-grade thermal cameras.
  • To facilitate the use of affordable thermal imaging in reproducible scientific research.

Main Methods:

  • IRimage processes raw thermal image data using an open and documented algorithm.
  • The software calculates temperature values and enables reproducible measurements across image series.
  • It integrates with ImageJ, offering a graphical user interface and script-based extensibility.

Main Results:

  • IRimage demonstrated consistency with standard software across 15 camera models.
  • The tool successfully processed hundreds of thermal images to detect subtle differences in plant leaf temperature.
  • Visual outputs (images, videos) suitable for scientific reporting are generated.

Conclusions:

  • IRimage provides a reliable and accessible solution for analyzing thermal images from consumer-grade cameras.
  • Its features enhance suitability for research purposes compared to many alternatives.
  • The software contributes to the utilization of affordable thermal imaging for reproducible scientific investigations.