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Updated: Sep 21, 2025

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

Published on: June 7, 2018

Murine brain tumor microenvironment immunophenotyping using mass cytometry.

Brandon L McClellan^1,2, Mahmoud S Alghamri^1,2, Rohit Thalla^1,2

¹Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI 48109, USA.

|May 31, 2022

Summary

This summary is machine-generated.

This study details a mass cytometry protocol for analyzing immune cells in mouse glioma. The optimized method aids in understanding the tumor microenvironment in brain cancer research.

Keywords:

Antibody Cancer Cell Biology Flow Cytometry/Mass Cytometry Immunology Model Organisms Neuroscience Single Cell

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Enrichment and Characterization of the Tumor Immune and Non-immune Microenvironments in Established Subcutaneous Murine Tumors

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Published on: June 7, 2018

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Expanding the Comprehension of the Tumor Microenvironment using Mass Spectrometry Imaging of Formalin-Fixed and Paraffin-Embedded Tissue Samples

Published on: June 29, 2022

Area of Science:

Immunology
Oncology
Neuroscience

Background:

The glioma microenvironment harbors diverse immune cells crucial for tumor progression.
Understanding immune cell phenotypes is vital for developing effective brain cancer therapies.

Purpose of the Study:

To present an optimized mass cytometry protocol for dissecting immune cell phenotypes in mouse glioma models.
To provide a comprehensive guide for antibody conjugation, tissue processing, and data analysis.

Main Methods:

Utilized a Sleeping Beauty transposon-mediated mouse glioma model.
Developed and optimized antibody conjugation and titration strategies.
Detailed procedures for brain tumor tissue collection, processing, staining, data acquisition, and analysis.

Main Results:

Established a robust mass cytometry protocol applicable to various brain tumor mouse models.
Enabled detailed phenotypic analysis of immune cells within the glioma microenvironment.

Conclusions:

The presented protocol offers a standardized approach for immune cell profiling in brain tumors.
Facilitates in-depth investigation of the immune landscape in glioma research.