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Genetic Polymorphisms of Pneumocystis jirovecii in HIV-Positive and HIV-Negative Patients in Northern China

Ting Xue1, Wei-Qin Du2, Wen-Juan Dai1

  • 1NHC Key Laboratory of Pneumoconiosis, Key Laboratory of Prophylaxis and Treatment and Basic Research of Respiratory Diseases of Shanxi Province, Department of Respiratory and Critical Care Medicine, First Affiliated Hospital of Shanxi Medical University, Taiyuan, P.R. China.

Polish Journal of Microbiology
|May 31, 2022
PubMed

Insights

Genetic analysis of Pneumocystis jirovecii in pneumonia cases revealed mixed infections in two of three patients. No drug resistance mutations were found in this initial Shanxi Province study.

Area of Science:

  • Medical Mycology
  • Infectious Diseases
  • Genetics

Background:

  • Pneumocystis jirovecii causes severe Pneumocystis pneumonia (PCP) in immunocompromised individuals.
  • Understanding P. jirovecii genetic diversity is crucial for tracking its epidemiology and treatment.

Purpose of the Study:

  • To investigate the genetic polymorphisms of P. jirovecii in PCP patients from Shanxi Province, China.
  • To analyze genetic variations at nuclear and mitochondrial loci and assess potential drug resistance markers.

Main Methods:

  • PCR amplification and sequencing of eight P. jirovecii gene loci (six nuclear, two mitochondrial).
  • Analysis of samples from three PCP patients (two HIV-positive, one HIV-negative).

Main Results:

  • Coinfections with multiple P. jirovecii genotypes were observed in two patients (one HIV-negative, one HIV-positive) at specific loci.
  • The third patient (HIV-positive) presented a single genotype infection across all analyzed loci.
  • No mutations associated with drug resistance were detected in key drug target genes (dhfr, dhps, cyb).

Conclusions:

  • This study provides the first report on P. jirovecii genetic polymorphisms in PCP patients in Shanxi Province.
  • Findings highlight the presence of genetic diversity and coinfections of P. jirovecii in this region.
  • Further research is needed to understand the clinical implications of P. jirovecii genetic diversity in China.
Keywords:
Pneumocystis jiroveciiepidemiologygenetic polymorphismsgenotypesmultilocus

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