Aluminum uptake, translocation, physiological changes, and overall growth inhibition in rice genotypes (Oryza sativa)...

Aluminum (Al) contamination in acidic soil is a major problem in paddy field, causing grain yield loss, especially in central plains of Thailand. The objective of this study was to assess Al content in the root tissues, its translocation to the leaves, and Al toxicity in three genotypes of rice, RD35 (local acidic-tolerant), Azucena (positive-check Al-tolerant), and IR64 (high yielding) under 0 (control) or 1 mM AlCl₃ (Al toxicity) at pH 4.5. Al content in the root tissues of rice cv. RD35 under 1 mM AlCl₃ was peaked at 4.18 mg g^‒1 DW and significantly translocated to leaf tissues (0.35 mg g^‒1 DW), leading to reduced leaf greenness (SPAD) (by 44.9% over the control) and declined net photosynthetic rate (P_n) (by 54.5% over the control). In contrast, Al level in cvs. Azucena and IR64 was restricted in the roots (2.12 mg g^‒1 DW) with low amount of translocation in the leaf tissues (0.26 mg g^‒1 DW), resulting in maintained values of SPAD and P_n. In cv. RD35, root and shoot traits including root length, root fresh weight, shoot height, shoot fresh weight, and shoot dry weight in 1 mM Al treatment were significantly dropped by > 35% over the control, whereas these parameters in cvs. Azucena and IR64 were retained. Based on the results, RD35 rice genotype was identified as Al sensitive as it demonstrated Al toxicity in both aboveground and belowground parts, whereas Azucena and IR64 were found tolerant to 1 mM Al as they demonstrated storage of Al in the root tissues to reduce toxicity in the leaf tissues. The study suggests that root traits, shoot attributes, chlorophyll degradation, and photosynthetic reduction can be successfully employed for the screening of Al-tolerant genotypes in rice breeding programs.