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Updated: Sep 21, 2025
An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
Published on: May 23, 2018
Fast and highly sensitive full-length single-cell RNA sequencing using FLASH-seq
Vincent Hahaut1,2, Dinko Pavlinic1,2, Walter Carbone3
1Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.
Abstract:
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples.
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